LEPR CLIA Kit for Pig(Sus scrofa) | EIAab.comLEPR CLIA Kit for Pig(Sus scrofa) | EIAab.com

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LEPR (Gene name),

Leptin receptor (Protein name ), LEPR_PIG from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

LEPR(OBR);

Protein name:

Leptin receptor(LEP-R);

Alternative:

OB receptor(OB-R);

CD:

CD295;

Organism:

Pig (Sus scrofa).

General Annotation

Sub Unit:

On leptin stimulation, homodimerizes (By similarity). The phosphorylated receptor binds a number of SH2 domain-containing proteins such as STAT3, PTPN11, and SOCS3 (By similarity). Interaction with SOCS3 inhibits LRb signaling.

Function:

Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3 (By similarity). Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway.

Subcellular Location:

Membrane Single-pass type I membrane protein

Protein Attributes:

Sequence length:

1165

Sequence:

50:

MTCPKFSVAL | LHWEFIYVIT | AFDLAYPITP | WKFKLSCMPP | NTTYDFLLPA |

100:

GISKNTSTLN | GHDEAVVETE | LNSSGTYLSN | LSSKTTFHCC | FWSEEDKNCS |

150:

VHADNIAGKA | FVSAVNSLVF | QQTGANWNIQ | CWMKEDLKLF | ICYMESLFKN |

200:

PFKNYDLKVH | LLYVLLEVLE | GSPLLPQKGS | FQSVQCNCSA | RECCECHVPV |

250:

SAAKLNYTLL | MYLKITSGGA | VFHSPLMSVQ | PINVVKPDPP | LGLHMEITDT |

300:

GNLKISWSSP | TLVPFQLQYQ | VKYSENSTTN | MREADEIVSD | TSLLVDSVLP |

350:

GSSYEVQVRG | KRLDGPGIWS | DWSTPFTFTT | QDVIYFPPKI | LTSVGSNISF |

400:

HCIYKNENKI | VSSKKIVWWM | NLAEKIPQSQ | YDVVGDHVSK | VTFPNMNATK |

450:

PRGKFTYDAV | YCCNEHECHH | RYAELYVIDV | NINISCETDG | YLTKMTCRWS |

500:

TNAIQSLVGS | TLQLRYHRSS | LYCSDVPSVH | PISEPKDCQL | QRDGFYECIF |

550:

QPIFLLSGYT | MWIRINHPLG | SLDSPPTCVI | PDSVVKPLPP | SSVKAEITAK |

600:

IGLLKISWEK | PVFPENNLQF | QIRYGLSGKE | VQWKIYEVYD | TKLKSTSLPV |

650:

PDLCAVYAVQ | VRCKRLDGLG | YWSNWSTPAY | TVVTDVKVPI | RGPEFWRIIN |

700:

EDATKKERNI | TLLWKPLMKN | DSLCSVRSYV | VKHHTSRHGT | WSEDVGNHTK |

750:

LTFLWTEQAH | SVTVLAVNSI | GASSANFNLT | FSWPMSKVNI | VQSLSAYPLN |

800:

SSCVGLSWLL | SPSDYNLMYF | ILEWKILNED | HEIKWLRIPS | SVKKYYIHDH |

850:

FIPIEKYQFS | LYPIFMEGVG | KPKIINSFTQ | DGEKHRNDAG | LYVIVPIIIS |

900:

SSILLLGTLL | MSHQRMKKLF | WEDVPNPKNC | SWAQGLNFQK | PETFEHLFIK |

950:

HTESVTFGPL | LLEPETISED | ISVDTSWKNK | DEMVPPTTVS | LLLTTPDLEK |

1000:

SSICISDQRS | SAHFSEAESM | EITREDENRR | QPSIKYATLL | SSPKSGETEQ |

1050:

EQELVSSLVS | RCFSSSNSLP | KESFSNSSWE | IETQAFFILS | DQHPNMTSPH |

1100:

LSFSEGLDEL | MKFEGNFPKE | HNDERSVYYL | GVTSIKKRES | DVFLTDESRV |

1150:

RCPFPAHCLF | ADIKILQESC | SHLVENNFNL | GTSGQKTFVS | YMPQFQTCST |

1165:

QTQKIMENKM | YDLTV

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Pfam:

KEGG:

Uniprot:

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ELISA Kit for Pig LEP-R

Cat.:

E0083m

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Manual:

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MSDS:

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Packing:

96T

Range:

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ELISA Kit for Pig LEP-R

Cat.:

E0083p

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MSDS:

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Packing:

96T

ELISA Kit for Pig LEP-R

Cat.:

E0083h

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Manual:

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96T

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ELISA Kit for Pig LEP-R

Cat.:

E0083r

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MSDS:

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Packing:

96T

CLIA Kit for Pig LEP-R

Cat.:

U0083r

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Pig LEP-R

Cat.:

U0083p

Price:

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MSDS:

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Packing:

96T

CLIA Kit for Pig LEP-R

Cat.:

U0083h

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Manual:

Looking for manual.

MSDS:

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Packing:

96T

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CLIA Kit for Pig LEP-R

Cat.:

U0083m

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MSDS:

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Packing:

96T

Polyclonal Antibody for Pig LEP-R

Cat.:

P0083Rb-h

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig LEP-R

Cat.:

P0083Rb-r

Price:

Please sign in first.

Packing:

40ug/0.2ml

Polyclonal Antibody for Pig LEP-R

Cat.:

P0083Rb-m

Price:

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Packing:

40ug/0.2ml

Polyclonal Antibody for Pig LEP-R

Monoclonal Antibody for Pig LEP-R

Monoclonal Antibody for Pig LEP-R

Monoclonal Antibody for Pig LEP-R

Monoclonal Antibody for Pig LEP-R

Protein for Pig LEP-R

Protein for Pig LEP-R

Protein for Pig LEP-R

Protein for Pig LEP-R

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

s

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.6%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.9%

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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Recovery

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Recovery was determined by spiking various levels of LEPR into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	93-101
Plasma	98	95-102

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Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

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For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LEPR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-99%	95-100%	93-102%	92-103%
Saliva(n=3)	91-103%	93-102%	94-100%	93-101%
heparin plasma(n=5)	95-100%	91-104%	95-100%	94-100%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
Saliva(n=3)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

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References

1.

"Porcine leptin receptor: molecular structure and expression in the ovary."
Ruiz-Cortes Z.T. , Men T. , Palin M.-F. , Downey B.R. , Lacroix D.A. , Murphy B.D.
Mol. Reprod. Dev.56:465-474(2000) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
tissue: Liver.
2.

"Porcine leptin receptor intron 3, partial."
Lacroix D.A. , Gevry N.Y. , Ruiz-Cortes Z.T. , Murphy B.D.
Submitted (1999-09) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-123
strain: Yorkshire X Meishan.
3.

"Expression, detection, and partial cloning of porcine leptin receptor (OBR) gene."
Hu X. , Dai R. , Li N. , Wu C.
Submitted (1999-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-854
tissue: Liver.
4.

"Partial cDNA sequence of the porcine leptin receptor."
Matteri R.L. , Carroll J.A.
Submitted (1997-12) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-159 AND 916-1088
tissue: Hypothalamus.
5.

"HpaII and RsaI PCR-RFLPs within an intron of the porcine leptin receptor gene (LEPR) and its linkage mapping."
Stratil A. , Kopecny M. , Moser G. , Schroffel J. Jr. , Cepica S.
Anim. Genet.29:405-406(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 109-123
6.

"The leptin receptor gene (LEPR) maps to porcine chromosome 6."
Ernst C.W. , Kapke P.A. , Yerle M. , Rothschild M.F.
Mamm. Genome8:226-226(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 408-470

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